ELISA Analyzer

Processing high-throughput samples, intelligent reuse for large-capacity publishing, work surface: 200cm, 8 sample injection needles, 12 temperature-controlled incubation positions, 12 room temperature incubation positions, 32 plate storage positions, Sunrise microplate reader, HydroFlex plate washer, up to 512 specimens, sequential loading of samples, reagents, microplates Parallel loading of up to 6 plates for fast dispensing.

The automatic enzyme immunoassay analyzer is based on the principle that the enzyme and the substrate can produce a color reaction, the absorption lines of different substances have different characteristics, and strictly abide by the Lambert-Beer law, quantitative and qualitative analysis of substances. instrument. The method of analyzing the content of various enzymes such as antigen or antibody generally mainly adopts colorimetric method. In practice, spectrophotometry is the basic working principle of an automatic enzyme immunoassay analyzer. The light emitted by the light source lamp becomes a beam of monochromatic light after passing through a filter or a monochromator. The monochromatic light beam passes through the sample to be tested in the microtiter plate, and part of the monochromatic light beam is absorbed by the sample and reaches the photodetector. The intensity of the light signal projected on it is converted into the magnitude of the electrical signal by the photodetector. This electrical signal is processed by pre-amplification, logarithmic amplification, analog-to-digital conversion, etc., and then sent to the microprocessor for data processing and calculation, and the test results are output by the display and printer. The microprocessor completes the movement in the X and Y directions of the mechanical drive through the control circuit.
The automatic enzyme immunoassay analyzer adds the sample to the microwells of the pre-coated antigen or antibody microtiter plate, washes after the reaction, removes the unseparated ligand, then adds the enzyme isolate, after incubation, washes again , remove the unseparated compound, and then add the enzyme substrate, after the reaction, the colored final product is formed, and the stop solution is added to stop the reaction. The absorbance of each microwell of the microtiter plate is read by the wavelength that has been set by the spectrophotometer. The concentration value of the analyte in the sample is calculated by the absorbance value of the sample and the standard curve, so that the quantitative result can be obtained, or the absorbance of the sample is compared with that of the standard product, so that the positive or negative qualitative result can be obtained.